Part:BBa_K4496003:Design

Optimized Bj46a for Escherichia coli

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 1059
  Illegal SpeI site found at 60
  Illegal PstI site found at 1194
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 19
  Illegal NheI site found at 42
  Illegal SpeI site found at 60
  Illegal PstI site found at 1194
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 1059
  Illegal SpeI site found at 60
  Illegal PstI site found at 1194
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 1059
  Illegal SpeI site found at 60
  Illegal PstI site found at 1194
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Using bioinformatics tools, the best DNA gene sequence corresponding to the mRNA of the original part (UniProtKB/Swiss-Prot: Q9DGI0.1) was found. With this, The signal peptide was removed, the sequence was optimized for synthesis using the bacteria Escherichia coli, promotor and terminator and RBS have been added. The promoter used was BBa_J23100, the RBS used was BBa_J61101 and the terminator used was BBa_B0015.

Source

https://www.ncbi.nlm.nih.gov/protein/Q9DGI0.1

References

BASTOS, Viviane de Almeida. CARACTERIZAÇÃO DA PORÇÃO GLICÍDICA DE BJ46a, UM INIBIDOR DE METALOPROTEINASES DE VENENOS DE SERPENTES. 2014. 122 f. Dissertation (Master Degree) - Course of Cellular and Molecular Biology, Oswaldo Cruz Institute, Rio de Janeiro, 2014.

PALACIO, Tatiane Zapata. ISOLAMENTO E CARACTERIZAÇÃO BIOQUÍMICA E FUNCIONAL DE UM INIBIDOR DE METALOPROTEASE PRESENTE NO SORO DA SERPENTE Bothrops alternatus. 2014. Dissertation (Master Degree) - Sciences Course, University of São Paulo, Ribeirão Preto, 2014.

VALENTE, R. H. et al. BJ46a, a snake venom metalloproteinase inhibitor. European Journal of Biochemistry, v. 268, n. 10, p. 3042–3052, May 15, 2001.